Real-time, High-density Monitoring of pTyr Signaling Targets in Human Tumors using SureQuant Heavy Peptide Triggered Targeted Quantitation
I was fortunate enough to be able to present our collaborative work using the SureQuant method and the new Exploris 480 Orbitrap Mass Spectrometer at the 2019 Thermo Scientific Mass Spec Users’ Meeting in Somerset, New Jersey.
This work leverages the SureQuant internal standard parallel reaction monitoring (IS-PRM) method built into the Exploris. We use SureQuant to selectively monitor 350 unique tyrosine phosphorylation sites to profile the tyrosine phosphorylation signaling networks in 30 human colon cancer samples. This work reveals the unique tyrosine signatures among patients, and suggests targets for therapy by looking for enrichment of tyrosine mediated signaling networks.
The work was presented at the users’ meetings in Cambridge by my advisor, Forest White and by my colleauge Cameron Flower in New York as well. Many thanks to our collaborators at Thermo Scientific, especially Aaron Gajadhar who worked with us on this exciting, cutting edge project, as well as Adreas Huhmer for inviting me to present, and the organizing team of the 2019 Mass Spec Users meetings. What an opportunity to connect with the northeast mass spec community!